VIROTYPING KIT InfA H5N8

VIROTYPING KIT InfA H5N8

Product description

Product description:

ViroTyping Kit InfA H5N8 contains two PCR primer sets for the amplification and detection of RNA of avian influenza A H5N8. This test is based on an intercalating dye using one-step reverse-transcription real-time PCR and was developed and validated for the Applied Biosystems® 7500 (Fast) instrument (Thermo Fisher Scientific), but is also suitable for other real-time PCR instruments. This test allows the rapid typing of avian influenza H5N8 from RNA samples.
ViroTyping Kit InfA H5N8 detects the haemagglutinin (H) and neuraminidase (N) genes of avian influenza A virus, subtype H5N8. Amplification curves and specific melting curves for the targets H5 and N8 indicate the amplification of genotype specific RNA.
When using PCR-platforms not validated by ingenetix, an evaluation of the real-time PCR is recommended. Please be aware that some PCR-platforms have to be calibrated with the corresponding dye before performing PCR.

Pathogen information:

Influenza A virus is a single-stranded, negative-sense RNA virus of the family Orthomyxoviridae. Of the three Influenza genera (A, B, C), only Influenza A is known to infect birds. Avian Influenza is further characterized based on the presence of haemagglutinin/neuroaminidase subtypes. Various strains, especially of H5N1 and H5N8 genotype, are responsible for outbreaks in domestic ducks and migratory birds in East Asia, Northern America, parts of Europe and the Middle East. Further spreading around the globe is possible through migratory birds. Virulence potential of a certain strain can vary from mild to severe due to high sequence variability.

Principle of Real Time PCR:

When detecting pathogens by reverse transcription real-time PCR, a specific RNA sequence of the pathogen genome is transcribed into cDNA and amplified. The generated PCR-product is detected by an intercalating nucleic acid dye with subsequent melt curve analysis.